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Image Search Results
Journal: Cells
Article Title: Generation of Functional Vascular Endothelial Cells and Pericytes from Keratinocyte Derived Human Induced Pluripotent Stem Cells
doi: 10.3390/cells10010074
Figure Lengend Snippet: Co-culture of human induced pluripotent stem cells (hiPSC)-derived endothelial cells (ECs) and pericytes. ( a ). Co-cultured hiPSC-derived ECs and pericytes form blood vessel-like structures in vitro under 2D culturing conditions. Fluorescent imaging shows blood vessel-like structures that contain ECs (positively stained for CD31) and pericytes (positively stained for Platelet Derived Growth Factor Receptor β (PDGFR-β)); scale bars: 100 μm. ( b ). Co-cultured hiPSC-derived ECs and pericytes form a 3D microvascular network in the chip. Live-cell imaging, both ECs, and PCs express mCherry for visualization; scale bars: 1 mm ( c ). Fluorescent imaging shows the 3D microvascular network in the chip, ECs and PCs express mCherry, ECs are positively stained for CD31. White arrows show the colocalization of pericytes with ECs; scale bars: 100 μm.
Article Snippet: In the project,
Techniques: Co-Culture Assay, Derivative Assay, Cell Culture, In Vitro, Imaging, Staining, Live Cell Imaging